Abstract: To meet the technical requirements of dynamic environment test of spacecraft, a data acquisition and processing system is developed with 128 channels based on VXI bus. The block transfer mode and synchronous clock are used in its hardware design, and the object oriented method and the hierarchical function module management are used in its software design. The system features the high-speed synchronous sampling, real-time mass data transmission, parallel task realization, and good expandability. The system can be used in dynamic environment testing for spacecraft.