Abstract: Before conducting the ground microgravity simulation test on spacecraft, it is necessary to test the characteristics of flexible ropes to determine whether they meet the requirements of gravity unloading. In this paper, a method for testing the characteristics of flexible ropes was proposed. By conducting stiffness, frequency, and creep tests, its gravity unloading characteristics were obtained. In addition, the inconsistency between static frequency and dynamic frequency, as well as the influence of temperature on its dynamic frequency were discussed. The selection of flexible ropes was analyzed according to the simulation requirements of a specified microgravity environment. While satisfying the total load weight, flexible ropes with low longitudinal frequency, large single-rope load capacity, low space requirement, and short creep stability time were selected. The proposed study may provide a reference for the selection of flexible ropes in ground microgravity simulation experiments.