Abstract: In order to deeply analyze the influencing factors and their change laws of aerodynamic heat generation of centrifuge, a heat generation model was theoretically derived and established. The effectiveness of the theoretical model was verified by comparing the experimental and simulation results of the aerodynamic heat generation of centrifuge in the literature. The model was used to analyze and calculate the actual centrifuge heat generation. It is found that the air flow ratio in the chamber increases with the increase of rotor angular velocity ω_r in the range of working conditions studied. The total heat generation power increases exponentially with the rotor speed and is proportional to the chamber pressure. The friction heat generation power of the chamber side wall is the largest among all heat sources of the centrifuge and increases with the increase of ω_r. It is suggested that the heat exchanger of the centrifuge be arranged on the inner surface of the chamber side wall to artificially improve the air flow ratio in the chamber, so as to facilitate the heat dissipation and temperature control of the centrifuge.