Abstract: Based on the environment stress screening (EES) test requirement for the printed circuit board (PCB), an optimization method is proposed for the design of the PCB’s fixture with the constraints of the PCB size, the test efficiency and the natural frequency of the structure, to reduce the influences of the fixture on the test results. A certain type of PCB is taken as an example, and its fixture is designed with the iterative optimization. The effectiveness of the designed fixture is verified by the modal test analysis. The vibration transmission characteristics of the designed fixture are analyzed and the test scheme is then solidified. Based on the fixture and the determined program, the random vibration tests of the EES for the PCB are carried out. It is shown that the first order natural frequency of the designed fixture is higher than the upper limit of the specified test frequency band, thus the fixture meet the stiffness requirement. Through reasonable mounting and arrangement of sensors, the errors of the PSD curves of the acceleration and the root mean square (RMS) values at the control points can meet the relevant requirements of GJB 1032A.