Abstract: A method of the time domain waveform reproduction (TWR) of micro-vibration of multi-degrees of freedom is proposed to meet the requirement of the micro-vibration environment simulation for spacecraft. Firstly, the theory of the micro-vibration environment simulation based on the time domain waveform reproduction is discussed. Then, a multi-input-multi-output (MIMO) micro-vibration incitation system is established using the MATLAB software based on the transfer function matrix measured in test. The control program for the micro-vibration TWR is developed based on the flow chart of the closed-loop control. Finally, the noise that shows the nonlinear influence of system and measurement errors is added in the simulation examples. Simulation results demonstrate the feasibility and the effectiveness of the control method of the TWR, which could be used for the study of the micro-vibration TWR control of multi-degree of freedom.