Abstract: For the on-board micro-vibration of a spacecraft, the disturbance source and the micro-vibration effect on the space scientific experimental instruments and the earth-imaging payloads are analyzed. A typical configuration of the micro-vibration measurement unit is designed. A method of on-ground calibration for the scale factor and the offset value is proposed and verified by accurate alignment tests. The testing result shows that the measurement error in each axis is less than 5×10^-3g₀ and the design and the calibration method are feasible.