Abstract: In order to better control the microbes and to provide guidance for the design of the microbe control in manned spacecrafts in the future, the concentrations and the distribution characteristics of the airborne microbes in three different manned spacecraft AIT centers are measured by using the impacting method. The results show that by comparing the microbes in the three AIT centers, the bacterial level in the Beijing AIT center is significantly higher than that in the AIT of other regions (P<0.05), and the fungal level in the Tianjin AIT center is significantly higher than that in the AIT of other regions (P<0.05). In all three AIT centers, the total amount of microbes in the Jiuquan AIT is the largest (P<0.05). As for the species in AIT centers, in the Beijing AIT, the dominant bacteria are Micrococcus and Staphylococcus; and the dominant fungi are Candida albicans. In the Tianjin AIT center, the dominant bacteria are Bacillus, and the dominant fungi are Aspergillus and Penicillium. In the Jiuquan AIT center, the dominant bacteria are Bacillus and Staphylococcus, and the dominant fungi are Aspergillus. It is indicated that the distribution characteristics of air microbes in different regions are distinctly different, which are affected not only by climatic characteristics and workshop design, but also by human factors.This study provides some guidance on the microbe control in AIT centers of China's space station.